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KMID : 0360220160570010113
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Journal of the Korean Ophthalmological Society
2016 Volume.57 No. 1 p.113 ~ p.119
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Neuroprotective Effects of Betaxolol Mediated by Heme Oxygenase-1 Induction in RGC-5
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차재봉:Cha Jae-Bong
권민영:Kwon Min-Young/정수월:Chung Su-Wol/우제문:Woo Je-Moon
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Abstract
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Purpose: To evaluate the neuroprotective effects of betaxolol (betaxolol hydrochloride) under hypoxic conditions using retinal ganglion cells (RGC-5) and determine whether heme oxygenase-1 (HO-1) expression exerts cytoprotective effects.
Methods: In this study, cultured RGC-5 cells were incubated with different concentrations of betaxolol hydrochloride (0.1 μM, 1 μM or 5 μM) and with 10 μM zinc protoporphyrin (ZnPP), in a hypoxia incubator (1% O2, 5% CO2, 94% N2) for 48 hours and the cell viability of each group was determined. Additionally, cell viability was measured after RGC-5 cells were incubated with 5 μM of brinzolamide (Azoptⓡ), brimonidine tartrate (Alphaganⓡ) or travoprost (Travatanⓡ). RGC-5 cells were divided into three groups and incubated under three different conditions, normoxia group (20% O2, 5% CO2), hypoxia group (1% O2, 5% CO2) and the group with 5 μM of Betoptic Sⓡ treated under hypoxic conditions (hypoxia, Betoptic Sⓡ). After incubation for 4, 8, 12 and 24 hours, HO-1 expression was analyzed using Western blotting.
Results: Cell viability significantly increased in RGC-5 cells treated with Betoptic Sⓡ compared with other antiglaucoma agents. Increased levels of HO-1 expression indicate its relevance in cell viability. Furthermore, increased RGC-5 cell viability by Betoptic Sⓡ was significantly reduced in the HO-1 inhibitor ZnPP-treated group.
Conclusions: We reaffirmed the known cytoprotective effects of BetopticSⓡ and the results suggests that HO-1 expression exerts cytoprotective effects against hypoxia.
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KEYWORD
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Betaxolol, Heme oxygenage-1, Hypoxia, Retinal ganglion cells
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